Location:
4/5 Rimpa Plaza, M.A. Jinnah Rd.
Karachi, Pakistan
Course Fees: Rs. 10,500/-
Course Description:
Students will learn concepts of the HPLC, the utility of each working part and the effects it has on the separation of chemicals. This will ensure a better understanding of how the instrument puts chemistry to work, so that the students may fully understand the utility of the HPLC as a whole ensuring better operating skills and hence being able to produce better more consistent results.
Text: Will be provided at the time of fees collection
Course Outline:
- FUNDAMENTALS
- FUNDAMENTALS’ AND FACTORS EFFECTING AND CAUSING SEPARATION
- FLOW DIAGRAM OF HPLC ( function of hardware)
- HPLC SYSTEM MODES (pump)
- Injector (Comparison)
- HPLC MODES AND THEIR APPLICATIONS (Column)
- COLUMNS PARAMETERS
- DETECTORS
- DATA ANALYSIS
- QUANTIZATION
- Qualitative analysis
- Quantitative analysis
- HARDWARE CONFIGURATION AND ITS UTILIZATION
- HPLC System
- Pump
- Injector
- Column temperature controlling device
- Detectors (theory and laws)
- DATA PROCESSING
- Software ( Starting to result calibration report)
- MAINTENANCE AND TROUBLESHOOTING (precautions & handling)
- Routine maintenance (Degasser)
- Preliminary checks
- Tubing (tubing, fittings and its connection)
- Filtration and purging
- Sample pretreatment
- Mobile phase
- Column
- Injector
- Detectors
- Trouble shooting related to instrument
- Trouble shooting related to Chromatogram
- Strategies for best possible outcomes
- FUNDAMENTAL OPERATIONAL TECHNIQUES
- Connectivity of devices
- Purging
- Column installation
- Leakage checking
- Sample injection
- Sample preparation (TIPS)
Course Mechanics/Schedule:
Lecture sessions:
This course consists of six lecture sessions, each being four hours long.
Practical sessions:
After the first lecture the whole batch will be taken into the lab for a primilinary lab session. Then students will be divided into equal groups and will be appointed a date where they will perform hands on tasks (runs) in the lab.
Additional Information:
*tea and lunch will be complementary throughout the course |
HPLC (High Performance Liquid Chromotography)
